Flow Injection SPR
Flow injection (FI) SPR allows one to carry out SPR
analysis in a continuous liquid stream. The advantages
of FI include (1) high-throughput analysis, (2) enhanced
response times, (3) simple and compact instrumentation,
(4) little carry-over from run to run, (5) low sample
consumptions, and (6) quick start-up and prompt shut-down
times. Thus far, most bioaffinity studies involve
monitoring the analyte or target in solution with
the recognizing (probe) species immobilized onto the
SPR sensor chip. The injected sample plug is diverted
to the SPR flow cell by a carrier (buffer) solution,
while the change in the SPR signal is monitored as
a function of time. The resultant SPR-time curve (sensorgram)
can then be used to extract the binding constant between
the probe and the target. In many cases, a Langmuir
isotherm is used to simulate a series of SPR-time
curves obtained at different analyte concentrations.
The simulation will yield the association (ka) and
dissociation (kd) constants.
BI-SPR Technology
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Flow
Injection SPR •
Electrochemical
SPR •
Gas
Phase SPR
More on Flow Injection SPR: Choosing
a right flow rate.
