SPR Technology
Surface Plasmon Resonance (SPR) has
emerged as a powerful technique in life science research,
drug discovery, electroanalysis, food quality and
safety, environmental science and chemical sensor
development. The key of the technique is to excite
and detect collective oscillations of free electrons
in a metal film referred to as surface plasmons. The
excitation and detection can be achieved using several
different configurations. A widely used one is the
Kretschmann configuration, in which light is focused
onto the metal film through a glass prism and the
reflection of the light is detected
(Fig.
A). At a certain incident angle (resonance
angle), the plasmons are set to resonate with light,
resulting in absorption of light at that angle and
thus a dark line in the reflected beam
(Fig.
B). A molecular binding event taking place
on or near the metal film, or a conformational change
in the molecules bound to the film causes a shift
in the resonance angle. By monitoring the shift vs.
time, molecular binding events can be monitored and
kinetics of the binding events can be studied without
labels
(Fig. C).
BI-SPR Technology
.
Flow
Injection SPR .
Electrochemical
SPR
Gas Phase SPR .
Technical Notes
