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SPR Technology

Surface Plasmon Resonance (SPR) has emerged as a powerful technique in life science research, drug discovery, electroanalysis, food quality and safety, environmental science and chemical sensor development. The key of the technique is to excite and detect collective oscillations of free electrons in a metal film referred to as surface plasmons. The excitation and detection can be achieved using several different configurations. A widely used one is the Kretschmann configuration, in which light is focused onto the metal film through a glass prism and the reflection of the light is detected (Fig. A). At a certain incident angle (resonance angle), the plasmons are set to resonate with light, resulting in absorption of light at that angle and thus a dark line in the reflected beam (Fig. B). A molecular binding event taking place on or near the metal film, or a conformational change in the molecules bound to the film causes a shift in the resonance angle. By monitoring the shift vs. time, molecular binding events can be monitored and kinetics of the binding events can be studied without labels (Fig. C).

BI-SPR Technology . Flow Injection SPR . Electrochemical SPR
Gas Phase SPR . Technical Notes


 
  A                              
 
A. A light is incident upon a metal film through a prism and the reflected beam is collected and analyzed.
 
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Angular shift
       
B
B. The excitation of surface plasmons results in a dark line in the reflected beam, and the angular position of the dark line shifts as a molecule binding event takes place.
 
 
C. The angular shift vs. time provides a good study of the binding kinetics. The reverse process, molecular dissociation, can be studied in a similar way.
C
Angular shift
   
Detection of SPR

Since the amount of the angular shift is proportional to the number and size of the molecules, in order to detect the binding event of extremely dilute or small analyte molecules, a SPR instrument that can measure a very small angular shift is essential. For fast kinetics, one desires the instrument with a fast response time. Biosensing Instruments Inc. uses a unique detection scheme to achieve both high angular resolution and a fast sampling speed to assist users to maximize the potential of the SPR technique.
   
Biosensing Instrument, Inc. is a leading manufacturer of surface plasmon resonance instruments.
Biosensing Instrument surface plasmon resonance instruments are technically innovative.
A surface plasmon resonance instrument from Biosensing Instrument is made in the United States.
Biosensing Instrument sells a biosensing instrument that uses surface plasmon resonance as its method of detection.
Contact Biosensing Instrument soon for all your biosensing instrument needs.


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